OBTENTION OF RABIES ANTIGEN THROUGH BHK21 CELLS ADHERED TO MICROCARRIERS

A rabies virus persistent infection in BHK21 cells.

A rabies virus persistent infection in BHK21 S13 cells was established and maintained in culture for more than 4 years. Initially, the cultures produced a large plaque virus similar to that produced by the original virus, but between the 10th and 20th passage, this was replaced by a small plaque variant. By the 200th passage, infectious virus could no longer be detected in the medium. After fur...

The aggregation of trypsinized BHK21 cells.

The aggregation in suspension of freshly trypsinized cultured cells (BHK21) has been studied by microscopic and electronic counting techniques. Comparison of cells grown in suspension with cells grown on glass suggests that the aggregation may not be an artifact of tryptic action, but may reflect an adhesive property of the cell surface which survives the dispersal procedure. The aggregation is...

The effect of neuraminidase on the aggregation of BHK21 cells and BHK21 cells transformed by polyoma virus.

Bacterial neuraminidases increase the adhesiveness in suspension of freshly trypsinized hamster fibroblasts (BHK21 cells). The effect is seen most clearly when the enzymes are added to pre-aggregated suspensions, but can also be observed when cells in monolayer culture are pre-incubated with neuraminidase, suspended, and then aggregated in its absence. BHK21 cells transformed by polyoma virus r...

Time to appreciate Avicenna’s knowledge of rabies

On the reduced intercellular adhesiveness of virally transformed BHK21 cells.

Baby hamster kidney fibroblasts (BHK21 cells) transformed by polyoma or Rous sarcoma viruses aggregate less than the untransformed parental cells when incubated in growth medium in a gyratory shaker for 18-24 h. This difference can be measured by electronic particle counting, or by filtering aggregated suspensions of 32P-labelled cells through bolting fabric. The aggregation of transformed deri...